Title:
Differential Analysis of Carbohydrate Content in Selected Rice Varieties Using the Anthrone Spectrophotometric Method

Authors:
Nattava Prasanna Lakshmi, Varshitha Kollipara, Manganuri Gnana Prasuna, Yanamandra Pujitha Vaishnavi, Palle Sai Teja, P. Rohan, India

Abstract:

Carbohydrates are essential macronutrients besides proteins and fats, by functioning as the primary source of energy and maintains the normal physiological processes. Both overconsumption or insufficient consumption of carbohydrates results in serious ailments such as diabetes, chronic kidney disease, etc. Therefore, evaluating carbohydrate content in staple foods like rice is essential for disease prevention and management. This study focuses on the estimation of carbohydrate content in several varieties of rice (Oryza sativa), namely brown rice, jasmine rice, sona masoori rice, basmati rice, black rice, fortified rice, red rice and quinoa. The qualitative estimation of carbohydrates was performed by the Molisch test and iodine test which aid in establishing the presence of carbohydrates in various sample extracts. Whereas the quantitative determination was executed by the anthrone method using colorimeter. Additionally, all of the samples were analysed by IR spectroscopy to further validate the efficiency of the tests performed. The anthrone method estimated the carbohydrate content as follows: (basmati rice-0.275 mg/ml, Sona Masoori-0.325 mg/ml, jasmine rice-0.613 mg/ml, fortified rice-0.5mg/ml, quinoa-0.475 mg/ml and brown rice-0.238 mg/ml). The IR spectra of the samples exhibited peak values at wavelengths that correspond to the functional groups associated with carbohydrates. The results of the anthrone method signified the nutritional diversity of the samples. These results were further validated by the findings of the IR spectra. Several health conditions can thus be managed by incorporating the suitable rice variety in accordance to their carbohydrate content.

DOI: http://dx.doi.org/10.51505/ijmshr.2026.10302